| dc.contributor.author | Mangare, Caroline | |
| dc.contributor.author | Tischer-Zimmermann, Sabine | |
| dc.contributor.author | Riese, Sebastian B. | |
| dc.contributor.author | et al. | |
| dc.date.accessioned | 2026-01-21T06:25:09Z | |
| dc.date.available | 2026-01-21T06:25:09Z | |
| dc.date.issued | 2019 | |
| dc.identifier.uri | 10.3390/ijms20061415 | |
| dc.identifier.uri | http://repository.mut.ac.ke:8080/xmlui/handle/123456789/7003 | |
| dc.description.abstract | Viral infections and reactivations remain a serious obstacle to successful hematopoietic
stem cell transplantation (HSCT). When antiviral drug treatment fails, adoptive virus-specific T-cell
transfer provides an effective alternative. Assuming that naive T cells (TN) are mainly responsible
for GvHD, methods were developed to generate naive T-cell-depleted products while preserving
immune memory against viral infections. We compared two major strategies to deplete potentially
alloreactive T cells: CD45RA and CD62L depletion and analyzed phenotype and functionality
of the resulting CD45RA−/CD62L− naive T-cell-depleted as well as CD45RA+/CD62L+ naive
T-cell-enriched fractions in the CMV pp65 and IE1 antigen model. CD45RA depletion resulted in
loss of terminally differentiated effector memory T cells re-expressing CD45RA (TEMRA), and CD62L
depletion in loss of central memory T cells (TCM). Based on these differences in target cell-dependent
and target cell-independent assays, antigen-specific T-cell responses in CD62L-depleted fraction were
consistently 3–5 fold higher than those in CD45RA-depleted fraction. Interestingly, we also observed
high donor variability in the CD45RA-depleted fraction, resulting in a substantial loss of immune
memory. Accordingly, we identified donors with expected response (DER) and unexpected response
(DUR). Taken together, our results showed that a naive T-cell depletion method should be chosen
individually, based on the immunophenotypic composition of the T-cell populations present | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | International Journal of Molecular Sciences | en_US |
| dc.subject | cytomegalovirus (CMV); donor lymphocyte infusions (DLIs); graft versus host disease (GvHD); naive T-cell depletion | en_US |
| dc.title | Robust Identification of Suitable T-Cell Subsets for Personalized CMV-Specific T-Cell Immunotherapy Using CD45RA and CD62L Microbeads | en_US |
| dc.type | Article | en_US |
