The purpose of the study was to determine the effects of natural resources on tourism sustainability in Maasai Mara National Game Reserve, Kenya. The study adopted explanatory research design. The target population was 169,220 household in Narok County, 300 tourists and 18 experts. The sample size was 507 respondents comprising of 399 households, 90 tourists and 18 experts selected by simple random sampling, convenience sampling and snowball sampling respectively. Structured questionnaires collected data from the host community which was analyzed using Pearson product moment of correlation, linear and multiple regressions. Data analyses were done through quantitative techniques of descriptive and inferential statistics with the aid of SPSS V.23. The processes Macro were used to test the mediation effects. Results indicated that there was a positive significant relationship between climate change and natural resources (β=0.286 and p=0.000). Moreover, climate change affects natural resources positively as there was a positive significant relationship between natural resources and tourism sustainability (β=0.393 and p<0.000). The natural resources controlling for climate change predicts 42.3% of tourism sustainability. In conclusion, natural resources mediate the relationship between climate change and tourism sustainability. Despite moderate adaptive capacity to climate change effects, the study raises urgent interventions for the tourism industry stakeholders on rainfall and temperature variations that positively contribute to influence the decline in wildlife populations and natural vegetation. Tourism operators have no choice but to adopt mitigation and adaptation strategies. It is crucial that policies aimed at strengthening climate change mitigation and adaptation strategies should be enacted at the same time full implementation of the Climate Change Act of 2016. A need exists for future research to use other climate change indicators other than rainfall and temperature.