Most studies have shown that gender mainstreaming in South Sudan has not been well undertaken this is due to lack of working policy or lack of policy implementation. While expansive body of literature has explored the implications of gender disparities in South Sudan, none has been published on the impact National Gender Policy full implementation. This study aims at assessing the implementation of South Sudan National Gender Policy in Jubek State, South Sudan. The study was guided by three objectives namely; to identify the gender gaps in the National gender Policy as a step towards achieving gender equality in Jubek state in gender mainstreaming in Jubek State; and to determine stakeholders’ achievement in a national gender policy in Jubek State. The study employed a stand point theory. A population of 350 government officials and members of the public in the Jubek State targeted in the study with a randomly selected sample of 105 respondents. Members of the public responded to questionnaire while Government Officials to respond to both interview guide questionnaire. The aim of the study was to assess this strategies that help in the implementation of Gender policy in all government Ministries. The study employed descriptive and inferential analysis with the aid of Statistical Package for the Social Sciences and data presented by Charts and tables. The study further revealed that despite the existence of South Sudan National Gender Policy its implementation is still a not clear since the general public is not even aware of its existence and there is no awareness to the public on the importance of the South Sudan National Gender Policy. The study further concluded that there is no emphasis national gender policy in gender mainstreaming. Gender responsive training and retraining programs have not been established. There are also no adequate resources allocated for gender mainstreaming. The government doesn’t increase financial allocation for interventions to increase retention rates for girls and boys in schools. Thus the national gender policy in gender mainstreaming has not produced any success. The study findings further revealed that stakeholders in national gender policy have not achieved a lot. The study recommends that, there is need for the government state ministries to establish more strict measures to ensure there is gender equity in employment in both private and public service. There is also need for the government stakeholders to create awareness to the public on the importance and implementation of Sudan National Gender Policy (SSNGP). The government should allocate adequate resources for gender mainstreaming. The government also needs to increase financial allocation for interventions to increase retention rates for girls and boys in schools. Thus the national gender