Supply chain collaboration has become a critical focus for manufacturing firms facing increasing disruptions that threaten operational performance. In an increasingly volatile global market, supply chain disruptions have become a persistent challenge for manufacturing firms, particularly in developing economies. Geopolitical conflicts, economic uncertainties, logistical bottlenecks, and infrastructural inefficiencies have significantly impacted the supply chain networks for manufacturers, leading to increased operational costs, production delays, and decreased competitiveness. This study examines the role of supply chain collaboration on operational performance of manufacturing firms in Nairobi County, Kenya. Supply chain Collaboration strategies, including resource Sharing, strategic supplier partnerships, information sharing, incentive alignment and collaborative demand forecasting were assessed for their role on operational performance of manufacturing firms. This study employed a descriptive research design. Data were collected using structured questionnaires administered to 240 manufacturing firms. The gathered data were then analyzed with the Statistical Package for Social Sciences (SPSS), and Pearson correlation analysis revealed a strong, positive relationship between supply chain collaboration and the operational performance of these firms. The findings underscore the importance of fostering trust, transparency, and technological integration among supply chain partners to optimize resource utilization and responsiveness. The study recommends that manufacturing firms enhance supply chain collaboration to strengthen operational performance, improve competitiveness, and mitigate the impact of disruptions. These insights provide valuable guidance for industry leaders, policymakers, and researchers seeking to develop robust supply chain frameworks.