The study describes the antibacterial and antifungal effects of both CH2Cl2 and MeOH crude extracts, collected fractions and pure isolates of Conzya floribunda. The antimicrobial activity tests were carried out using agar diffusion method. In vitro tests using CH2Cl2 and MeOH extracts of C. floribunda showed anti-bacterial activities against Streptococcus pneumoniae, Staphylococcus aureus and Escherischia coli, and antifungal activities against Candida albicans, Trichophyton mentagrophytes and Microsporum gypsium. The antibacterial and antifungal principles from C. floribunda were found to be (24S)-ethylcholesta-5, 22E, 25-dien-3-O -b-glucoside and cyasterone from methanol extract, and 3-oxofriedooleanane and betullinic acid associated with CH2Cl2 extract. The results of the present study indicate that the plant could be a useful remedy for some of the disease conditions caused by the tested bacteria and fungi and the isolated compounds could be good